The classical crystal structure of insulin was determined in 1969 by D.C. Hodgkin et al. following a 35-year program of research. This structure depicted a hexamer remarkable for its self-assembly as a zinc-coordinated trimer of dimer. Prominent at the dimer interface was an "aromatic triplet" of conserved residues at consecutive positions in the B chain: PheB24 , PheB25 and TyrB26 . The elegance of this interface inspired the Oxford team to poetry: "A thing of beauty is a joy forever" (John Keats as quoted by Blundell, T.L., et al. Advances in Protein Chemistry 26:279-286 [1972]). Here, we revisit this aromatic triplet in light of recent advances in the structural biology of insulin bound as a monomer to fragments of the insulin receptor. Such co-crystal structures have defined how these side chains pack at the primary hormone-binding surface of the receptor ectodomain. On receptor binding, the B-chain β-strand (residues B24-B28) containing the aromatic triplet detaches from the α-helical core of the hormone. Whereas TyrB26 lies at the periphery of the receptor interface and may functionally be replaced by a diverse set of substitutions, PheB24 and PheB25 engage invariant elements of receptor domains L1 and αCT. These critical contacts were anticipated by the discovery of diabetes-associated mutations at these positions by Donald Steiner et al. at the University of Chicago. Conservation of PheB24 , PheB25 and TyrB26 among vertebrate insulins reflects the striking confluence of structure-based evolutionary constraints: foldability, protective self-assembly and hormonal activity.
A Thing of Beauty!
A Thing of Beauty is a novel by author A. J. Cronin, initially published in 1956, with the alternate title of Crusader's Tomb. It tells the story of Stephen Desmonde, an English painter who struggles for recognition in a conventional world, sacrificing everything for his passion for art. The title is a reference to John Keats' 1818 poem, Endymion, which begins with "A thing of beauty is a joy for ever."
NEW YORK -- After the Boston Celtics set a franchise record with 27 made 3-pointers in a 133-118 win here at Madison Square Garden over the New York Knicks, Celtics guard Marcus Smart called the team's offense "a thing of beauty."
"It felt amazing," Smart said. "(Celtics coach Joe Mazzulla) was just preaching to us, 'Don't get tired, we're playing the right way.' I think every coach preaches that, but to see it actually happen in action is a thing of beauty.
"It's good for us. It felt good for guys to hit shots, felt good for the team to see how we play when we play that way and the things we can do, so we have to keep preaching that, we have to keep working."
"That's a lot of threes," said Knicks guard RJ Barrett, who scored 27 points for New York (4-5). "Sometimes a team gets hot. Tough to beat 27 threes, but we still fought all the way through. Nothing we can do about that one."
THING OF BEAUTY Words & Music by Dave NachmanoffI'd like to make a sculpture out of glassSo beautiful, that it could make you cryBut when I look out at the snowflakesThat fall against my windowI cannot seem to bring myself to tryI just can't seem to bring myself to tryChorus:To make a thing of beautyJust one thing of beautyOnly for an instantWith truth and with loveTo make a thing of beautyJust one thing of beautyA single thing of beautyAnd that would be enoughI'd like to weave a tapestry of yarnSo intricate, that it could break your heartBut when I look out at that webAs it's shining in the sunlightI cannot seem to bring myself to startI just can't seem to bring myself to start(Repeat chorus)I'd like to sing a song of breath and airSo wonderful, that it could touch your soulBut when I listen to robinAs he's heralding the springtimeI cannot take the first step towards my goalI just can't take the first step towards my goalFinal Chorus:But I can try to live a lifeThat makes an honest storyWith challenges adventuresWith truth and with loveAnd that would be a thing of beautyA single thing of beautyA human thing of beauty And that would be enough
Everything about "OldOS" applies equally to AT&T Unix and to BeOS, and everything about NewOS applies to both Linux and to Haiku. In fact, the "NewOS" name itself was the original name for the Haiku kernel.
While it supports multiple users, it doesn't scream a multiuser OS experience: it boots straight into the desktop, with no login screen or anything, as the root or administrator user. Like a smartphone, it's your computer, so it trusts you to know what you're doing and not break it.
Lois Shaw has been a part of the PCC community for over 30 years, currently serving on the Advisory Council of Women (ACW). She loves nothing more than plunging into a good Bible Study, or enjoying a great book discussion. She is exhausted by a brisk walk and energized by a challenging Scrabble game. She is married to Mark, and enjoys serving with him as part of Peninsula Fellows. Lois has children and grandchildren in Gloucester, VA and Goma, Congo.
You just have to wonder how many more of these personalities coach Marvin Lewis can handle. Lewis and his staff have done a great job of managing a lot of players who have proved hard to work with in the past, but with Owens now in the mix, the cup could overflow if things don't materialize.
Lots of things failed in the garden. His entries from 1809 show the carrots, beets, sorrel and okra, the cauliflower, tarragon and Chinese melons missing the mark. Jefferson cites Windsor Beans as "killed by bug" and notes on Aug. 21: "From the 7th of Apr. to this day, excessive drought and cold. Now a good rain."
Despite the diversity of vegetables Jefferson's garden produced, the recipes unearthed by scholars and attributed to his family were quite typical for the day: Boil everything. Some of the recipes survived and were reprinted in The Congressional Cook Book (1933). If you're looking for instructions for Colonial American-style Cabbage Pudding and Dried Beans, check this out. They're hard to make out, but here are some written in Jefferson's own hand.
These stiletto heels are a thing of beauty! Step up your night-out attire in these so chic heels featuring an almond toe, open back, and a spiral rhinestone strap with crystal charms. Complete the look in a satin dress and clutch.
A great backstroke start is a thing of beauty. I liken it to a dolphin leaping out of the water or David Boudia scoring a perfect 10 off of the 10-meter tower. You see no splash and hear no splash. Current photo via TRC
A great backstroke start is a thing of beauty. I liken it to a dolphin leaping out of the water and piercing the water through a hula-hoop, or David Boudia, scoring a perfect 10 off of the 10-meter tower. You see no splash and hear no splash.
A math genius and engineering major in college, he's still working on this writing thing. He says that the three years he spent as an F.M. Black Panther probably played a more significant a role in the man he would become than the time he spent in college.
We are pleased to share with you, photos that our customers have shared with us. We would love to see your plants and florals, too! Send your photos of hanging baskets, planters, window boxes, bouquets and seasonal decor purchased through us to info@athingofbeauty.ca
"It's not going to be a fast process, there's no silver bullet, and I think as the organisations learn more about each other and their strengths and relative weaknesses, it's something that will grow over a period of time. But we're not actually putting any timescale on that."
"We already saw from Toro Rosso last year that great progress was made, and of course our decision was based on what we could see and measure. And I have to say so far, we've been very impressed with everything we've seen.
That last step is all important. Why the double-wrapped sandwiches? Sure, they look cool, but as every professional sandwich wrapper knows, it's all about preservation. Tinfoil locks in the heat, while parchment keeps things nice and tidy. You can wrap a sandwich super tight and open it up 20 minutes later to the same pull-y, cheesy goodness the people that actually showed up to the party on time enjoyed. That's called insurance.
I have a love/hate affair with the desert. I've lived all my life in desert regions and declared as a teenager that "One day I will move away from all the dirt." I long for cabins in the forest and grey foggy mornings. Yet here I am, still in the region of dirt. But I'd be remiss to say I don't love it too. I do. I love the mild winters, and I appreciate the scorching heat (in small doses). In late summer, I love the change of landscape when I catch a glimpse of my surroundings and think, "Oh, where did all the dirt go?" Mark took me for a drive early on in our days together. He pulled off a dusty road and beckoned me to follow where he pulled a branch of sticky, bright green leaves off a nearby Creosote bush. He cupped the branch in his hands and raised it to my nose. I inhaled deeply and there was only one word to describe that overwhelming aroma: rain. Fresh, earthy desert rain. A thing of beauty.I've wondered over the years why this plant is so precious to us desert dwellers. Why do we cherish it so? But, the reason is simple: memory and nostalgia. It reminds us of the fleeting piece of time we get in the summer when the desert treats us to its most dramatic show of power and strength. It's a vivid reminder that you're just an occupant on this spinning rock at the whim of something much larger. The power of nature has a way of putting things into perspective. 2ff7e9595c
Comments